AnaSpec Introduces New Kinase Peptide Library

September 7, 2007 – San Jose, CA - Recent articles by B. Turk et al.1-2 reported the use of a positional scanning synthetic peptide combinatorial libraries (PS-SPCL)3 for rapid identification of serine/threonine kinase phosphorylation motif. AnaSpec, the key supplier of kinase peptide libraries mentioned in the articles, has announced the worldwide commercial availability of its newest kinase peptide library.

Protein kinases play an important universal role in the regulation of signal transduction pathways by catalyzing the reversible phosphorylation of protein substrates. To date, 518 putative protein kinase genes have been identified, but not all have been characterized.4 Profiling the sequence preference of different kinases will help in understanding the roles played by the different kinases as well as identification of potential inhibitors.1

The library consists of 198 distinct peptide mixtures. “In each mixture, one of the 20 naturally occurring proteogenic amino acids was fixed at each of 9 positions surrounding a central serine/threonine residue.” 2


Product Size Catalog #
Kinase Substrates Library, Group I, biotinylated, 180 peptides
1mg x 180 vials 62017-1
Kinase Substrates Library, Group I, biotinylated, 180 peptides
5mg x 180 vials 62017-5
Kinase Substrates Library, Group II, biotinylated, 18 peptides
1mg x 18 vials 62335


The following shows how these peptides are configured:

Group I:
Y-A-Z-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH2, 20 peptides
Y-A-X-Z-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH2, 20 peptides
Y-A-X-X-Z-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH2, 20 peptides
Y-A-X-X-X-Z-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH2, 20 peptides
Y-A-X-X-X-X-Z-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH2, 20 peptides
Y-A-X-X-X-X-X-S/T-Z-X-X-X-A-G-K-K(LC-Biotin)-NH2, 20 peptides
Y-A-X-X-X-X-X-S/T-X-Z-X-X-A-G-K-K(LC-Biotin)-NH2, 20 peptides
Y-A-X-X-X-X-X-S/T-X-X-Z-X-A-G-K-K(LC-Biotin)-NH2, 20 peptides
Y-A-X-X-X-X-X-S/T-X-X-X-Z-A-G-K-K(LC-Biotin)-NH2, 20 peptides

X = degenerate mixture of the 17 natural amino acids excluding cysteine, serine, and threonine.
LC = "long chain" version with an additional aminohexanoic acid spacer between the biotin and lysine sidechain.
S/T = equimolar mixture of serine and threonine.
Z = one of the 20 naturally occurring amino acids fixed at this postiion

Group II:
Y-A-pT-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-pT-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-pT-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-pT-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-pT-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-pT-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-pT-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-X-pT-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-X-X-pT-A-G-K-K(LC-biotin)-NH2

Y-A-pY-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-pY-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-pY-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-pY-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-pY-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-pY-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-pY-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-X-pY-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-X-X-pY-A-G-K-K(LC-biotin)-NH2

X = degenerate mixture of the 17 natural amino acids excluding cysteine, serine, and threonine.
K(biotin) is Lysyl-biotin, the "long chain" version with an additional aminohexanoic acid spacer between the biotin and lysine sidechain.
S/T = equimolar mixture of serine and threonine. pT is phosphothreonine, and pY is phosphotyrosine.

Company Info

AnaSpec, Inc. is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and combinatorial chemistry. Established in 1993, AnaSpec's ISO9001:2000 certified headquarters and manufacturing facilities are located in San Jose, CA.

For more information, visit www.anaspec.com

References:
1. Turk, BB. et al. Nature Protocols 1, 375 (2006).
2. Hutti, JE. et al. Nature Methods 1, 27 (2004).
3. Pinilla, C. et al. Biotechniques 13, 901 (1992).
4. Manning, G. et al. Science 298, 1912 (2002).