ABRF Poster Presents Comparison of Fluorescent Peptide Synthesis Methods

April 2007 – Tampa, FL — At the 2007 annual conference of the Association of Biomolecular Resource Facilities, AnaSpec presented a technical poster that compared the synthesis of a fluorescent peptide using two types of resins. The results of the poster indicated that the use of an unprotected resin was just as effective as using a protected resin for the synthesis of a 5-FAM labeled peptide.

Many FRET (Fluorescent Resonance Energy Transfer) peptides, as well as non-FRET peptides, requiring C-terminal fluorescent labels can be accomplished using either solution phase or solid phase synthesis. In solid phase synthesis, the use of the fluorophore, 5-carboxyfluorescein (5-FAM) generates stable amide bonds. In order to help facilitate the synthesis of C-terminal fluorescent peptides, AnaSpec prepared and
performed comparison studies using two kinds of resins, Fmoc-Lys(5-FAM)-Rink Amide resin (I) and Fmoc-Lys[5-FAM(trt)]-Rink Amide resin (II), the latter contains a phenolic hydroxyl group protected with a trityl group. The results of these studies showed that resin (I) is adequate for synthesis of C-terminal fluorescent labeled peptide.

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